EIF1B, 1-113aa, Human

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01-1768-1B
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Proteins, Recombinant
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Product Name EIF1B, 1-113aa, Human
Background EIF1B is crucial for the scanning process in vitro. During the scanning process, EIF1B is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. This protein enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. Recombinant human EIF1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Eukaryotic translation initiation factor 1B, GC20
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF
Molecular Weight 13.3 kDa (117aa) confirmed by MALDI-TOF
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol,0.1M NaCl, 0.1mM PMSF
Purity > 95 % by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_005866
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Pestova TV., et al. (2002) Genes Dev. 16(22):2906-22.
Asano K., et al. (2000) Genes Dec. 14(19):2534-46.

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