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|Product Name||EIF4E, 1-217aa, Human|
|Background||EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
|Synonyms||CBP, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV|
|Molecular Weight||21.7kDa (189aa) confirmed by MALDI-TOF|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl, 0.1mM PMSF|
|Purity||> 85% by SDS-PAGE|
|NCBI Accession #||NP_001959|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Bendahmane A., et al. (2010) PLoS One. 5(6):e11313.
Pelletier J., et al. (2010) J Gen Virol. 91(Pt 5):1224-8.
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