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|Product Name||ETFB, 1-255aa, Human|
|Background||ETF (electron transfer flavoprotein) is a heterodimer of an alpha and beta subunit. The ETFB protein is electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Recombinant human ETFB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
|Synonyms||Electron transfer flavoprotein subunit beta, FP585, MADD|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MAELRVLVAV KRVIDYAVKI RVKPDRTGVV TDGVKHSMNP FCEIAVEEAV RLKEKKLVKE VIAVSCGPAQ CQETIRTALA MGADRGIHVE VPPAEAERLG PLQVARVLAK LAEKEKVDLV LLGKQAIDDD CNQTGQMTAG FLDWPQGTFA SQVTLEGDKL KVEREIDGGL ETLRLKLPAV VTADLRLNEP RYATLPNIMK AKKKKIEVIK PGDLGVDLTS KLSVISVEDP PQRTAGVKVE TTEDLVAKLK EIGRI|
|Molecular Weight||10.5kDa (94aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|Concentration||0.5mg/ml (determined by Bradford assay)|
|Form||Liquid, in 10mM sodium citrate (pH 3.5) , 10% glycerol|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_001976|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Colombo I., et al. (1994) Hum. Mol. Genet. 3:429-435
Bross P., et al. (1999) Mol. Genet. Metab. 67:138-147
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