FHL3, 1-280aa, Human

Catalog
01-P2411
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Proteins, Recombinant
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Product Name FHL3, 1-280aa, Human
Background FHL3 is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human FHL3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms SLIM2, NP_004459, SLIM-2, Skeletal muscle LIM-protein 2
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSMSESFDC AKCNESLYGR KYIQTDSGPY CVPCYDNTFA NTCAECQQLI GHDSRELFYE DRHFHEGCFR
CCRCQRSLAD EPFTCQDSEL LCNDCYCSAF SSQCSACGET VMPGSRKLEY GGQTWHEHCF LCSGCEQPLG SRSFVPDKGA HYCVPCYENK
FAPRCARCSK TLTQGGVTYR DQPWHRECLV CTGCQTPLAG QQFTSRDEDP YCVACFGELF APKCSSCKRP IVGLGGGKYV SFEDRHWHHN
CFSCARCSTS LVGQGFVPDG DQVLCQGCSQ AGP
Molecular Weight 30.8 kDa (291aa)
Concentration 0.5mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_004459
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Morgan M.J., et al. (1999) Biochem. Biophys. Res. Commun. 255:245-250
Morgan M.J., et al. (1996) Biochem. Biophys. Res. Commun. 225:632-638

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