GALM, 1-342aa, Human

Catalog
01-1875
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Proteins, Recombinant
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Product Name GALM, 1-342aa, Human
Background GALM (Galactose mutarotase), also known as Aldose 1-epimerase, is a key enzyme of carbohydrate metabolism catalysing the conversion of beta-D-galactose to alpha-D-galactose. This protein may be essential for normal galactose metabolism by maintaining the equilibrium of alpha and beta anomers of galactose. Also it is required for the production of complex oligosaccharides. Recombinant human GALM, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Synonyms Aldose 1-epimerase, BLOCK25, IBD1, Galctose mutarotase, Aldose1-epimerase, BLOCK 25, BLOCK-25
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MASVTRAVFG ELPSGGGTVE KFQLQSDLLR VDIISWGCTI TALEVKDRQG RASDVVLGFA ELEGYLQKQP YFGAVIGRVA NRIAKGTFKV DGKEYHLAIN KEPNSLHGGV RGFDKVLWTP RVLSNGVQFS RISPDGEEGY PGELKVWVTY TLDGGELIVN YRAQASQATP VNLTNHSYFN LAGQASPNIN DHEVTIEADT YLPVDETLIP TGEVAPVQGT AFDLRKPVEL GKHLQDFHLN GFDHNFCLKG SKEKHFCARV HHAASGRVLE VYTTQPGVQF YTGNFLDGTL KGKNGAVYPK HSGFCLETQN WPDAVNQPRF PPVLLRPGEE YDHTTWFKFS VA
Molecular Weight 22.1 kDa (198aa) confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 20% glycerol
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_620156
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Ross AC., et al. (2007) Biochemistry. 46(51):15198-207
Reece RJ., et al (2003) FEBS Lett. 543(1-3):21-4.

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