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|Product Name||GAMT, 1-236aa, Human|
|Background||Guanidinoacetate N-methyltransferase isoform a, also known as GAMT is a methyltansferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl domor. This enzyme participates in the two-step synthesis of the compound creatine from the protein building blocks glycine, arginine, and methionine. It is involved in providing energy for muscle contraction, and is also important in nervous system functioning. Also GAMT is active in the liver, pancreas, and kidneys. Recombinant human GAMT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||PIG2, TP53I2, Guanidinoacetate N-methyltransferase isoform a Guanidinoacetate N methyltransferase.|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MSAPSATPIF APGENCSPAW GAAPAAYDAA DTHLRILGKP VMERWETPYM HALAAAASSK GGRVLEVGFG MAIAASKVQE APIDEHWIIE CNDGVFQRLR DWAPRQTHKV IPLKGLWEDV APTLPDGHFD GILYDTYPLS EETWHTHQFN FIKNHAFRLL KPGGVLTYCN LTSWGELMKS KYSDITIMFE ETQVPALLEA GFRRENIRTE VMALVPPADC RYYAFPQMIT PLVTKG|
|Molecular Weight||45.9 kDa (408aa), confirmed by MALDI-TOF.|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_000147|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Almeida LS., et al. (2007) Mol Genet Metab. 91(1):1-6.
Jenne DE., et al. (1997) Biochem Biophys Res Commun. 238(3):723-7.
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