GAS7, 1-416aa, Human, Recombinant

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01-2116
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Proteins, Recombinant
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Product Name GAS7, 1-416aa, Human, Recombinant
Background Growth arrest-specific 7, also known as GAS7, is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development by promoting maturation and morphological differentiation of cerebellar neurons. Inhibition of GAS7 production in terminally differentiating cultures of embryonic murine cerebellum impedes neurite outgrowth. Conversely, the hyper-expression of GAS7 may play an important role in the initiation and development of human osteosarcoma. Recombinant GAS7, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Synonyms KIAA0394, Growth arrest-specific 7, isoform b Growth arrest specific 7, GAS 7, GAS7, MGC1348, MLL/GAS7 fusion protein.
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MKPGMVPPPP GEESQTVILP PGWQSYLSPQ GRRYYVNTTT NETTWERPSS SPGIPASPGS HRSSLPPTVN GYHASGTPAH PPETAHMSVR KSTGDSQNLG SSSPSKKQSK ENTITINCVT FPHPDTMPEQ QLLKPTEWSY CDYFWADKKD PQGNGTVAGF ELLLQKQLKG KQMQKEMSEF IRERIKIEED YAKNLAKLSQ NSLASQEEGS LGEAWAQVKK SLADEAEVHL KFSAKLHSEV EKPLMNFREN FKKDMKKCDH HIADLRKQLA SRYASVEKAR KALTERQRDL EMKTQQLEIK LSNKTEEDIK KARRKSTQAG DDLMRCVDLY NQAQSKWFEE MVTTTLELER LEVERVEMIR QHLCQYTQLR HETDMFNQST VEPVDQLLRK VDPAKDRELW VREHKTGNIR PVDMEI
Endotoxin Level < 1.0 EU per 1 microgram of protein (determined by LAL method)
Molecular Weight 14.1 kDa (128aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1mM DTT, 10% glycerol
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_958836
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Chao CC., et al. (2005). J Neurosci Res. 81(2):153-62
She BR., et al. (2002). Exp Cell Res. 273(1):34-44.

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