GATC, 1-136aa, Human

Catalog
01-P2183
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Proteins, Recombinant
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Product Name GATC, 1-136aa, Human
Background GATC allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Glutamyl-tRNA(Gln) amidotransferase, subunit C homolog, 15E1.2
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS
Molecular Weight 17.2 kDa (148aa), confirmed by MALDI-TOF
Concentration 0.25 mg/ml (determined by BCA assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol
Purity > 85% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_789788
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Nagao A., et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:16209-16214

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