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|Product Name||GCDH, 45-438aa, Human|
|Background||Glutaryl-CoA dehydrogenase(GCDH) belongs to the acyl-CoA dehydrogenase family. GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. This Protein uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. Recombinant GCDH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||ACAD5, GCD, Glutaryl-CoA dehydrogenase, ACAD-5, ACAD 5,|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGVSSVAY GLLARELERV DSGYRSAMSV QSSLVMHPIY AYGSEEQRQK YLPQLAKGEL LGCFGLTEPN SGSDPSSMET RAHYNSSNKS YTLNGTKTWI TNSPMADLFV VWARCEDGCI RGFLLEKGMR GLSAPRIQGK FSLRASATGM IIMDGVEVPE ENVLPGASSL GGPFGCLNNA RYGIAWGVLG ASEFCLHTAR QYALDRMQFG VPLARNQLIQ KKLADMLTEI TLGLHACLQL GRLKDQDKAA PEMVSLLKRN NCGKALDIAR QARDMLGGNG ISDEYHVIRH AMNLEAVNTY EGTHDIHALI LGRAITGIQA FTASK|
|Molecular Weight||42 kDa (361aa), confirmed by MALDI-TOF|
|Concentration||1mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_000150|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Keyser B., et al. (2008) Hum. Mol. Genet. 17:3854-3863
Westover JB., et al. (2001) Biochemistry. 40(46):14106-14.
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