| Product Name | GPD1, 1-349aa, Human |
|---|---|
| Description | GPD1, also known as glycerol-3-phosphate dehydrogenase, is an enzyme that catalyzes the reduction of dihydroxyacetone phosphate to sn-glycerol 3-phosphate. (sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH) Older terms for glycerol-3-phosphate dehydrogenase include alpha glycerol-3-phosphate dehydrogenase and glycerolphosphate dehydrogenase. However, GPD1 is not the same as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) whose substrate is an aldehyde not an alcohol. Recombinant GPD1 protein was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | , Glycerol-3-phosphate dehydrogenase [NAD+] AI747587, EC 1.1.1.8, FLJ26652, G3PD, Gdc-1, Gdc1, Gdp1, Glycerol 3 phosphate dehydrogenase 1, Glycerol 3 phosphate dehydrogenase cytosolic, Glycerol 3 phosphate dehydrogenase soluble, Glycerol-3-phosphate dehydrogenase, Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic, Glycerol-3-phosphate dehydrogenase 1 (soluble), Glycerol-3-phosphate dehydrogenase, soluble, Glycerphosphate dehydrogenase, GPD-C, Gpd1 protein, GPD2, Gpd3, Gpdc, GPDH, GPDH-C, Gpdhc, KIAA4010, MGC93453, MGPD, mKIAA4010. |
| Host | E.coli |
| Molecular Weight | 18.5kDa (168aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MASKKVCIVG SGNWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAEDADILI FVVPHQFIGK ICDQLKGHLK ANATGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVADEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC GALKNVVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGPVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE LLNGQKLQGP ETARELYSIL QHKGLVDKFP LFMAVYKVCY EGQPVGEFIH CLQNHPEHM |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Albertyn J., et al. (1992) FEBS Lett. 308 (2): 130-2. Ou X., et al. (2006) J. Mol. Biol. 357(3):858-69 |
| Supplier | ARP |
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