GPD1, 1-349aa, Human

Catalog
01-1788-1
$0.00
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Proteins, Recombinant
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Product Name GPD1, 1-349aa, Human
Background GPD1, also known as glycerol-3-phosphate dehydrogenase, is an enzyme that catalyzes the reduction of dihydroxyacetone phosphate to sn-glycerol 3-phosphate. (sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH) Older terms for glycerol-3-phosphate dehydrogenase include alpha glycerol-3-phosphate dehydrogenase and glycerolphosphate dehydrogenase. However, GPD1 is not the same as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) whose substrate is an aldehyde not an alcohol. Recombinant GPD1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms , Glycerol-3-phosphate dehydrogenase [NAD+] AI747587, EC 1.1.1.8, FLJ26652, G3PD, Gdc-1, Gdc1, Gdp1, Glycerol 3 phosphate dehydrogenase 1, Glycerol 3 phosphate dehydrogenase cytosolic, Glycerol 3 phosphate dehydrogenase soluble, Glycerol-3-phosphate dehydrogenase, Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic, Glycerol-3-phosphate dehydrogenase 1 (soluble), Glycerol-3-phosphate dehydrogenase, soluble, Glycerphosphate dehydrogenase, GPD-C, Gpd1 protein, GPD2, Gpd3, Gpdc, GPDH, GPDH-C, Gpdhc, KIAA4010, MGC93453, MGPD, mKIAA4010.
Species Human
Amino Acid Sequence MASKKVCIVG SGNWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAEDADILI FVVPHQFIGK ICDQLKGHLK ANATGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVADEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC GALKNVVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGPVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE LLNGQKLQGP ETARELYSIL QHKGLVDKFP LFMAVYKVCY EGQPVGEFIH CLQNHPEHM
Molecular Weight 18.5kDa (168aa), confirmed by MALDI-TOF.
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_005267
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Albertyn J., et al. (1992) FEBS Lett. 308 (2): 130-2.
Ou X., et al. (2006) J. Mol. Biol. 357(3):858-69

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