| Product Name | GroES, Recombinant |
|---|---|
| Description | GroES protein is the co-chaperonin of GroES in E.coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E.coli and cloned into an expression vector. This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques. |
| Synonyms | CPN10, GroES, 10 kDa chaperonin, groS, mopB, 10 kDa heat shock protein mitochondrial, Chaperonin 10, Cpn 10, Early pregnancy factor, EPF, Heat shock 10kD protein 1 chaperonin 10, Heat shock 10kDa protein 1, Heat shock 10kDa protein 1 chaperonin 10, HSP 10, HSP10, HSPE 1, HSPE1, |
| Host | E.coli |
| Molecular Weight | 55.7kDa (491 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA |
| Tag | His-tag |
| Reactivity | E. coli |
| Applications | SDS-PAGE |
| Form | Liquid, in 50 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Jue D. Wang, et al (1998) Proc. Natl. Acad. Sci. USA 95, 12163-12168 Landry,S.J., et al(1993) Nature 364, 255-258 Xu, Z., et al(1997) Nature 388,741-750 |
| Supplier | ARP |
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