GSTT1, 1-240aa, Human, Recombinant

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01-2040
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Proteins, Recombinant
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Product Name GSTT1, 1-240aa, Human, Recombinant
Background Glutathione S-transferase theta 1(GSTT1) is one of the GSTs' four main classes: alpha, mu, pi, theta. This enzyme is involved in activation and detoxification reactions and catalyzes the conjugation of industrial chemicals, e.g. epoxybutane, ethylene oxides, halomethane with glutathione. The absence of the GSTTI enzyme, might determine the individual risk for development of acquired aplastic anemia and acute myeloid leukemia. Recombinant human GSTT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Synonyms Glutathione S-transferase theta 1, GSTT1, GST class-theta-1, Glutathione transferase T1-1, Glutathione S-transferase theta 1 EC 2.5.1.18, Glutathione S transferase 5, Glutathione S transferase theta 1, GST class theta 1, Glutathione transferase T1 1, GST 5 5, GST CL1,
Species Human
Amino Acid Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMGL ELYLDLLSQP CRAVYIFAKK NDIPFELRIV DLIKGQHLSD ACAQVNPLKK VPALKDGDFT LTESVAILLY LTRKYKVPDY WYPQDLQARA RVDEYLAWQH TTLRRSCLRA LWHKVMFPVF LGEPVSPQTL AATLAELDVT LQLLEDKFLQ NKAFLTGPHI SLADLVAITE LMHPVGAGCQ VFEGRPKLAT WRQRVEAAVG EDLFQEAHEV ILKAKDFPPA DPTIKQKLMP WVLAMIR
Molecular Weight 27.6 kDa (247 aa), confirmed by MALDI-TOF.
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH7.0) containing 30% glycerol, 1mM EDTA, 0.1mM PMSF.
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # AAH07065
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Faramawy MM., et al. (2009). J Crit Care. 24(3):e7-10.
Delpisheh A., et al. (2009). Eur J Obstet Gynecol Reprod Biol. 143(1):38-42.

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