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|Product Name||HBZ, 1-142aa, Human, Recombinant|
|Background||Hemoglobin subunit zeta (HBZ) is belongs to the globin family. Zeta-globin is alpha-like hemoglobin. The HBZ polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The HBZ gene includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. Recombinant human HBZ fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||Zeta-globin, HBAZ, HBZ2, Hemoglobin subunit zeta HBZ 2, Hemoglobin zeta, Hemoglobin zeta chain, Zeta globin.|
|Amino Acid Sequence||MSLTKTERTI IVSMWAKIST QADTIGTETL ERLFLSHPQT KTYFPHFDLH PGSAQLRAHG SKVVAAVGDA VKSIDDIGGA LSKLSELHAY ILRVDPVNFK LLSHCLLVTL AARFPADFTA EAHAAWDKFL SVVSSVLTEK YRLEHHHHHH|
|Molecular Weight||78.4 kDa (677aa), confirmed by MALDI-TOF.|
|Concentration||0.25 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.15M NaCl|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_005323|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Lau ET., et al. (2001) Prenat. Diagn. 21(7): 529-39.
Kidd RD., et al. (2001) Biochemistry. 40(51):15669-75.
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