HBZ, 1-142aa, Human, Recombinant

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01-1813
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Proteins, Recombinant
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Product Name HBZ, 1-142aa, Human, Recombinant
Background Hemoglobin subunit zeta (HBZ) is belongs to the globin family. Zeta-globin is alpha-like hemoglobin. The HBZ polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The HBZ gene includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. Recombinant human HBZ fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Zeta-globin, HBAZ, HBZ2, Hemoglobin subunit zeta HBZ 2, Hemoglobin zeta, Hemoglobin zeta chain, Zeta globin.
Species Human
Amino Acid Sequence MSLTKTERTI IVSMWAKIST QADTIGTETL ERLFLSHPQT KTYFPHFDLH PGSAQLRAHG SKVVAAVGDA VKSIDDIGGA LSKLSELHAY ILRVDPVNFK LLSHCLLVTL AARFPADFTA EAHAAWDKFL SVVSSVLTEK YRLEHHHHHH
Molecular Weight 78.4 kDa (677aa), confirmed by MALDI-TOF.
Concentration 0.25 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.15M NaCl
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_005323
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Lau ET., et al. (2001) Prenat. Diagn. 21(7): 529-39.
Kidd RD., et al. (2001) Biochemistry. 40(51):15669-75.

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