| Product Name | Hif-1a (530-826aa) Human, Recombinant |
|---|---|
| Description | Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1beta subunit and one of three subunits (Hif-1alpha, Hif-2 or Hif-3). The activation of Hif-1 is closely associated with a variety of tumors and oncogenic pathways. Hif-1alpha consists of DNA binding domain(DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains(TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains. Hif-1 alpha (530-826 residues) contains two TAD and inhibitory domain. Recombinant Hif-1 alpha (530-826 residues) was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | HIF1A, MOP1, Hif-1 alpha (530-826 aa), HIF-1 alpha, HIF1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, Hypoxia-inducible factor 1 alpha, Hypoxia-inducible factor-1a Basic helix loop helix PAS protein MOP1, ARNT interacting protein, bHLHe78, HIF 1A, HIF 1alpha, HIF1 A, Hypoxia inducible factor 1 alpha, Hypoxia inducible factor 1 alpha isoform I.3, Hypoxia inducible factor 1 alpha subunit, Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor, Member of PAS protein 1, Member of PAS superfamily 1, Member of the PAS Superfamily 1, MOP 1, PASD 8, PASD8, |
| Host | E.coli |
| Molecular Weight | 21.8kDa (197aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris (pH 8.0) , 100mM NaCl |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Okuyama H. et al., (2006) J. Biol Chem. 281(22):15554-63 Berra E. et al., (2006) EMBO.7(1):41-5 |
| Supplier | ARP |
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