His-PP2Calpha (His-tagging Protein phosphatase 2C), Human, Recombinant

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01-2220
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Proteins, Recombinant
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Product Name His-PP2Calpha (His-tagging Protein phosphatase 2C), Human, Recombinant
Background Protein phosphatase 2C(PP2C alpha) is a Mn2+- or Mg2+- dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2C alpha (amino acids 1-382) was cloned into an E. coli expression vector. PP2C alpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.
Synonyms PDP1, PDP, PPM2C, PP2C alpha, Protein phosphatase 2C, PDP 1, EC 3.1.3.43, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, PPM1A, Protein phosphatase 1A (formerly 2C), ppm1a, FLJ42306, MGC9201, PP2CA, PP2Calpha, PP2C-alpha, protein phosphatase Mg2+/Mn2+ dependent 1A
Species Human
Amino Acid Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
Endotoxin Level < 1.0 EU per 1 microgram of protein (determined by LAL method)
Molecular Weight 13.4 kDa (130 aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris pH 7.5, 0.1 M NaCl
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_066283
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Das, A. K. et al. (1996) EMBO J. 15, 6798-6809

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