HIV1-p24, 155-321aa, HIV-1

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01-P0671
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Proteins, Recombinant
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Product Name HIV1-p24, 155-321aa, HIV-1
Background The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly, budding maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Synonyms CA, Capsid protein p24, Group-specific antigen - p24
Species HIV-1
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MWVKVVEEKA FSPEVIPMFS ALSEGATPQD LNTMLNTVGG HQAAMQMLKE TINEEAAEWD RLHPVHAGPI APGQMREPRG SDIAGTTSTL QEQIGWMTHN PPIPVGEIYK RWIILGLNKI VRMYSPTSIL DIRQGPKEPF RDYVDRFYKT LRAEQASQEV KNWMTETL
Molecular Weight 39.7 kDa(357aa), confirmed by MALDI-TOF
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol
Purity > 80% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # AAA44987
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Bernard NF., et al. (2009) Clin Immunol. 131(2):277-87.
Wagstaff M., et al. (1995) Immunology. 86(1):85-96.

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