| Product Name | HMGB2, 1-209aa, Human, Baculovirus |
|---|---|
| Description | High mobility group protein B2, also known as HMGB2, is a member of the non-histone chromosomal high-mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. Recombinant human HMGB2 protein, fused to His-tag at N-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques. |
| Synonyms | High mobility group protein B2, HMG2 |
| Host | E.coli |
| Molecular Weight | 37.6 kDa (342aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH TGSMGKGDPN KPRGKMSSYA FFVQTCREEH KKKHPDSSVN FAEFSKKCSE RWKTMSAKEK SKFEDMAKSD KARYDREMKN YVPPKGDKKG KKKDPNAPKR PPSAFFLFCS EHRPKIKSEH PGLSIGDTAK KLGEMWSEQS AKDKQPYEQK AAKLKEKYEK DIAAYRAKGK SEAGKKGPGR PTGSKKKNEP EDEEEEEEEE DEDEEEEDED EE |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Bustin M., et al. (1990) Biochem Biophys Acta. 1049:231-243. Shirakawa H., et al. (1992) J Biol Chem. 267:6641-6635. |
| Supplier | ARP |
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