HSD17B14, 1-270aa, Human

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01-1807-14
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Proteins, Recombinant
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Product Name HSD17B14, 1-270aa, Human
Background HSD17B14, also known as 17-beta-hydroxysteroid dehydrogenase 14, belongs to the 17-beta-HSD family of proteins, which regulate the availability of steroids within various tissues rough out the body. It exists as a homotetramer that localizes to the cytoplasm and is highly expressed in brain, placenta, liver and kidney. Recombinant human HSD17B14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Synonyms 17-beta-hydroxysteroid dehydrogenase 14, DHRS10, retSDR3, SDR47C1,
Species Human
Amino Acid Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATG TRYAGKVVVV TGGGRGIGAG IVRAFVNSGA RVVICDKDES GGRALEQELP GAVFILCDVT QEDDVKTLVS ETIRRFGRLD CVVNNAGHHP PPQRPEETSA QGFRQLLELN LLGTYTLTKL ALPYLRKSQG NVINISSLVG AIGQAQAVPY VATKGAVTAM TKALALDESP YGVRVNCISP GNIWTPLWEE LAALMPDPRA TIREGMLAQP LGRMGQPAEV GAAAVFLASE ANFCTGIELL VTGGAELGYG CKASRSTPVD APDIPS
Molecular Weight 14.9 kDa (131aa) confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 0.2M NaCl, 0.5mM EDTA.
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_057330
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Grimwood J., et al. (2004), Nature., 428: 529-535.
Lukacik P., et al. (2007). Biochem. J., 402: 419-427

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