LYPLA1, 1-230aa, Mouse

Catalog
01-1754-1
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Proteins, Recombinant
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Product Name LYPLA1, 1-230aa, Mouse
Background LYPLA1 is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS and also has depalmitoylating activity. Recombinant mouse LYPLA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Synonyms Acyl-protein thioesterase 1, lysophospholipase 1, Pla1a, APT-1, LysoPLA I, LPL-I, APT1
Species Mouse
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID
Molecular Weight 31.2 kDa (294aa), confirmed by MALDI-TOF
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl 1mM DTT, 10% glycerol
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_032892
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Schaloske R.H., et al. (2006) Biochim Biophys Acta. 1761:1246-1259.
Hiraoka M., et al. (2002) J. Biol. Chem 277:10090-10099.

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