MECR, 54-373aa, Human

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01-1866
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Proteins, Recombinant
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Product Name MECR, 54-373aa, Human
Background MECR(Mitochondrial trans-2-enoyl-CoA reductase), also known as NRBF1, catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH dependent manner with preference to medium chain length substrate. This protein has a role in the mitochondrial synthesis of fatty acids. Recombinant human MECR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Synonyms NRBF1, CGI-63, FASN2B, Trans-2-enoyl-CoA reductase, mitochondrial, HsNrbf1, CGI 63, Homolog of yeast 2 enoyl thioester reductase, NBRF 1, NBRF1, NRBF 1, NRBF1, Nuclear receptor binding factor 1
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MPAKVVELKN LELAAVRGSD VRVKMLAAPI NPSDINMIQG NYGLLPELPA VGGNEGVAQV VAVGSNVTGL KPGDWVIPAN AGLGTWRTEA VFSEEALIQV PSDIPLQSAA TLGVNPCTAY RMLMDFEQLQ PGDSVIQNAS NSGVGQAVIQ IAAALGLRTI NVVRDRPDIQ KLSDRLKSLG AEHVITEEEL RRPEMKNFFK DMPQPRLALN CVGGKSSTEL LRQLARGGTM VTYGGMAKQP VVASVSLLIF KDLKLRGFWL SQWKKDHSPD QFKELILTLC DLIRRGQLTA PACSQVPLQD YQSALEASMK PFISSKQILT M
Molecular Weight 24.8 kDa (221aa) confirmed by MALDI-TOF
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # AAH01419
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Scicluna EA., et al. (2010) Eur J Clin Microbiol Infect Dis. 29(2):163-70.
Chen ZJ., et al. (2008) J Mol Biol. 379(4):830-44.

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