MED21, 1-144aa, Human

Catalog
01-P2118
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Proteins, Recombinant
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More Information
Product Name MED21, 1-144aa, Human
Background MED21 is a member of the mediator complex subunit 21 family. The protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. Recombinant human MED21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Mediator of RNA polymerase II transcription subunit 21, SRB7, SURB7
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSMADRLTQ LQDAVNSLAD QFCNAIGVLQ QCGPPASFNN IQTAINKDQP ANPTEEYAQL FAALIARTAK DIDVLIDSLP SEESTAALQA ASLYKLEEEN HEAATCLEDV VYRGDMLLEK IQSALADIAQ SQLKTRSGTH SQSLPDS
Molecular Weight 43.1 kDa (396aa), confirmed by MALDI-TOF
Concentration 0.25 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_004255
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Baumli,S., et al. (2005) J. Biol. Chem. 280 (18), 18171-18178
Maldonado,E., et al. (1996) Nature 381 (6577), 86-89

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