| Product Name | METAP1D, 20-335aa, Human |
|---|---|
| Description | Methionine aminopeptidase 1D, mitochondrial precursor, also known as METAP1D, is a 335 amino acid mitochondrial protein that belongs to the peptidase M24A family. It is overexpressed in colon cancer cell lines, suggesting a role in tumorigenesis. METAP1D has also been found to remove methionine from the N-terminus of nascent proteins. METAP1D binds two cobalt ions per subunit and is encoded by a gene that maps to human chromosome 2q31.1. Recombinant human METAP1D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | MAP1D, Metap1l |
| Host | E.coli |
| Molecular Weight | 34.6kDa (304aa) |
| Amino Acid Sequence |
MGSSHHHHHH SSGLVPRGSH MGSSSPLNHI YLHKQSSSQQ RRNFFFRRQR DISHSIVLPA AVSSAHPVPK HIKKPDYVTT GIVPDWGDSI EVKNEDQIQG LHQACQLARH VLLLAGKSLK VDMTTEEIDA LVHREIISHN AYPSPLGYGG FPKSVCTSVN NVLCHGIPDS RPLQDGDIIN IDVTVYYNGY HGDTSETFLV GNVDECGKKL VEVARRCRDE AIAACRAGAP FSVIGNTISH ITHQNGFQVC PHFVGHGIGS YFHGHPEIWH HANDSDLPME EGMAFTIEPI ITEGSPEFKV LEDAWTVVSL DNQRSAQFEH TVLITSRGAQ ILTKLPHEA |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Leszczyniecka M., et al. (2006) Oncogene. 25: 3471-3478. Serero A., et al. (2003) J Biol Chem. 278: 52953-52963. |
| Supplier | ARP |
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