MGMT, 1-207aa, Human, Recombinant

Proteins, Recombinant
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Product Name MGMT, 1-207aa, Human, Recombinant
Background O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques.
Synonyms O-6-methylguanine-DNA methyltransferase, MGMT, EC, O-6-methylguanine-DNA-alkyltransferase, O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, MGC107020, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase.
Species Human
Endotoxin Level < 1.0 EU per 1 microgram of protein (determined by LAL method)
Molecular Weight 24.8 kDa (229 aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris pH7.5, 10% Glycerol
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_002403
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Matsukura S., et al. (2001) Annals of Surgical Oncology, 8(10):807--816
Esteller M.,et al. (2002) J Natl Cancer Inst. 2, 26-32.

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