Mimitin, 1-169aa, Human

Proteins, Recombinant
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Product Name Mimitin, 1-169aa, Human
Background NDUFAF2, also known as Mimitin, belongs to the complex I NDUFA12 subunit family. NADH dehydrogenase is an enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). It is the 'entry enzyme' of oxidative phosphorylation in the mitochondria. Mimitin protein acts as a molecular chaperone for mitochondrial complex I assembly. Recombinant human NDUFAF2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2, Mimitin, MMTN, NDUFA12L
Species Human
Molecular Weight 53.8kDa (494aa)
Concentration 1mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl, 0.1mM PMSF
Purity > 85% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_777549
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Tsuneoka M., et al (2005) J. Biol. Chem. 280:19977-19985
Ogilvie I., et al (2005) J. Clin. Invest. 115:2784-2792

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