| Product Name | NIP7, 1-180aa, Human |
|---|---|
| Description | NIP7, also known as the 60S ribosome subunit biogenesis protein NIP7 homolog, belongs to NIP7 family and contains 1 PUA domain. It interacts with pre-ribosome complex and may bind to RNA. This protein is required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly. Recombinant human NIP7 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | CGI-37, HSPC031, KD93, 60S ribosome subunit biogenesis protein NIP7 homolog 60S ribosome subunit biogenesis protein NIP7 homolog, CGI 37, CGI37, FLJ10296, HSPC180, KD93, NIP 7, Nuclear import 7, Nuclear import 7 homolog (S, cerevisiae), OK/SW cl.76, OK/SW cl.78. |
| Host | E.coli |
| Molecular Weight | 34.6 kDa (321aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MRPLTEEETR VMFEKIAKYI GENLQLLVDR PDGTYCFRLH NDRVYYVSEK IMKLAANISG DKLVSLGTCFGKFTKTHKFR LHVTALDYLA PYAKYKVWIK PGAEQSFLYG NHVLKSGLGR ITENTSQYQG VVVYSMADIPLGFGVAAKST QDCRKVDPMA IVVFHQADIG EYVRHEETLT LEHHHHHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Sekiguchi T., et al. (2004) J. Biol. Chem. 279(9):8343-50. Hesling C., et al. (2007) Exp. Cell. Res. 313(20):4180-95. |
| Supplier | ARP |
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