NUDT10, 1-164aa, Human

Catalog
01-1884-10
$0.00
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Proteins, Recombinant
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Product Name NUDT10, 1-164aa, Human
Background NUDT10, also known as APS2 or DIPP3alpha, is a member of the Nudix hydrolase family of pyrophosphatases. Nudix hydrolases contain a characteristic Nudix domain and are responsible for catalyzing the hydrolysis of nucleoside diphosphate derivatives. It functions as a manganese-dependent polyphosphate phosphohydrolase with an optimum pH of 8.5. This protein specifically metabolizes diadendosine-polyphosphates and, to a lesser extent, diphosphoinositol polyphosphates. Recombinant human NUDT10 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.
Synonyms Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, APS2, DIPP3a, hDIPP3alpha
Species Human
Amino Acid Sequence MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG GMEPEEEPGG AAVREVYEEA GVKGKLGRLL GVFEQNQDPK HRTYVYVLTV TELLEDWEDS VSIGRKREWF KVEDAIKVLQ CHKPVHAEYL EKLKLGGSPT NGNSMAPSSP DSDPLEHHHH HH
Molecular Weight 35.8 kDa(325aa) confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_694853
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Hidaka K. et al. (2002) J. Biol. Chem. 277: 32730-32738
Hua L.V. et al. (2003) Biochem. J. 373: 81-89

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