| Product Name | NUDT16, 1-195aa, Human |
|---|---|
| Description | NUDT16 is nuclear nucleoside diphosphatase proteins localized within foci in the nucleolus and nucleoplasm. This is a RNA-decapping enzyme that binds specifically to U8 snoRNA. It can remove m7G and m227G caps from RNAs, rendering them substrates for 5'-3' exonucleases for degradation in vivo. Recombinant human NUDT16 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Nudix type motif 16, |
| Host | E.coli |
| Molecular Weight | 26.7 kDa (238aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MAGARRLELG EALALGSGWR HACHALLYAP DPGMLFGRIP LRYAILMQMR FDGRLGFPGG FVDTQDRSLE DGLNRELREE LGEAAAAFRV ERTDYRSSHV GSGPRVVAHF YAKRLTLEEL LAVEAGATRA KDHGLEVLGL VRVPLYTLRD GVGGLPTFLE NSFIGSAREQ LLEALQDLGL LQSGSISGLK IPAHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.1M Nacl, 1mM DTT, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Peculis B.A., et al. (2007) J. Biol. Chem. 282:24792-24805 Ghosh T., et al. (2004) Mol. Cell 13:817-828 |
| Supplier | ARP |
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