PDAP1, 1-181aa, Human

Proteins, Recombinant
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Product Name PDAP1, 1-181aa, Human
Background PDGFA associated protein 1, also known as PDAP1, enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB. And its expression is induced by TNF-alpha, and cells overexpressing PDAP1 show significantly less apoptosis on exposure to TNF-alpha. Recombinant human PDAP1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms HASPP28, PAP, PAP1, PDGFA associated protein 1
Species Human
Molecular Weight 45.7 kDa (398aa) confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_055706
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Zahedi R.P., et al. (2008) J. Proteome Res. 7:526-534
Malka D., et al. (2000) Gastroenterology. 119(3):816-28.

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