PDE6D, 1-150aa, Human

Proteins, Recombinant
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Product Name PDE6D, 1-150aa, Human
Background Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alphabeta) that is regulated by two inhibitory gamma subunits. As PDE6D does not modify the catalytic properties of PDEalphabeta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms PDED, Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta, phosphodiesterase 6D, cGMP-specific, rod, delta
Species Human
Molecular Weight 19.4 kDa (173aa), confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_002592
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Zhang H., et al, (2007) Proc Natl Acad Sci U S A. 104(21):8857-62.
Dekomien., G. et al. (2003) Genet Sel Evol. 35(4):445-56.

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