PECI, 1-364aa, Human

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01-1864
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Proteins, Recombinant
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Product Name PECI, 1-364aa, Human
Background PECI, also referred to as DRS1, ACBD2 or HCA88, is a protein that localizes to the peroxisomal matrix and contains one ACB (acyl-CoA-binding) domain. This protein is expressed abundantly in liver, heart and skeletal muscle. It functions to catalyze the isomerization of both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species, playing an important role in the beta-oxidation of unsaturated fatty acids. Recombinant human PECI protein, fused to his-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms ACBD2, DRS1, HCA88, Peroxisomal 3,2-trans-enoyl-CoA isomerase, acyl Coenzyme A binding domain containing 2, D3 D2 enoyl CoA isomerase, DBI related protein 1, Delta(3) delta(2) enoyl CoA isomerase, Dodecenoyl CoA isomerase, DRS 1, HCA 88, Hepatocellular carcinoma associated antigen 88, Peroxisomal D3 D2 enoyl CoA isomerase, Renal carcinoma antigen NY REN 1
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MNRTAMRASQ KDFENSMNQV KLLKKDPGNE VKLKLYALYK QATEGPCNMP KPGVFDLINK AKWDAWNALG SLPKEAARQN YVDLVSSLSP SLESSSQVEP GTDRKSTGFE TLVVTSEDGI TKIMFNRPKK KNAINTEMYH EIMRALKAAS KDDSIITVLT GNGDYYSSGN DLTNFTDIPP GGVEEKAKNN AVLLREFVGC FIDFPKPLIA VVNGPAVGIS VTLLGLFDAV YASDRATFHT PFSHLGQSPE GCSSYTFPKI MSPAKATEML IFGKKLTAGE ACAQGLVTEV FPDSTFQKEV WTRLKAFAKL PPNALRISKE VIRKREREKL HAVNAEECNV LQGRWLSDEC TNAVVNFLSR KSKL
Molecular Weight 48.4kDa (433aa) confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_006108
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Dongyan Zhang,et al. (2002) J. Biol. Chem. 277: 9127-9132.

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