| Product Name | PFN2, 1-140aa, Human, Recombinant |
|---|---|
| Description | PFN2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein is ubiquitously expressed with highest expression in kidney, brain and skeletal muscle. Like other members of the profilin family, PFN2 functions as an actin monomer-binding protein that influences the structure of the cytoskeleton by regulating actin polymerization in response to extracellular signals. At high concentrations, it prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Recombinant PFN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | PFL, Profilin 2 D3S1319E, PFN 2, PFN2, Profilin II, ProfilinII, Profilin2 |
| Host | E.coli |
| Molecular Weight | 13.9 kDa (125aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPIEIDM IVGKDREGFF TNGLALGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline pH7.4 |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Gieslmann R., et al. (1995) Eur J Bilchem. 229(3):621-8. Reinhard M., et al. (1995) EMBO J. 14(8):1583-9. |
| Supplier | ARP |
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