PGLS, 1-258aa, Human

Catalog
01-PGLS
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Proteins, Recombinant
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Product Name PGLS, 1-258aa, Human
Background 6-phosphogluconolactonase, also known PGLS, is an enzyme in the second step pentose phosphate pathway, which is essential for the synthesis of nucleotide sugars and NADPH, the main source of reducing power. It converts 6-phosphogluconolactone to 6-phosphogluconate. Recombinant human PGLS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms 6-phosphogluconolactonase, 6PGL
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV PFEKHSTL
Molecular Weight 49.4 kDa (430aa) confirmed by MALDI-TOF
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_036220
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Collard F., et al. (1999) FEBS Lett. 459(2):223-6.
Hong M K., et al. (2008) Acta Crystallogr Sect F Struct Biol Cryst Commun. 64(11):1016-8.

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