PHF5A, 1-110aa, Human

Catalog
01-P2167
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Proteins, Recombinant
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Product Name PHF5A, 1-110aa, Human
Background PHF5A is a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. PHF5A contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. Recombinant human PHF5A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms PHD finger-like domain-containing protein 5A, PHD finger protein 5A, bK223H9.2, INI, Rds3, SAP14b, SF3b14b
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSMAKHHPD LIFCRKQAGV AIGRLCEKCD GKCVICDSYV RPCTLVRICD ECNYGSYQGR CVICGGPGVS DAYYCKECTI QEKDRDGCPK IVNLGSSKTD LFYERKKYGF KKR
Molecular Weight 13.2kDa (120aa)
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Purity > 85% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_116147
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Wagner SA, et al. (2011) Mol Cell Proteomics, PMID 21890473.

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