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|Product Name||Phosphoserine phosphatase Human, Recombinant|
|Background||Human Phosphoserine phosphatase (hPSP) is an important enzyme in the phosphorylated pathway of serine biosynthesis, which contributes a major portion of the endogenous L-serine. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2+ General references: -dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. Recently, its complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important to substrate recognition and hydrolysis. Recombinant human hPSP was overexpressed in E. coli and purified by conventional chromatography.|
|Synonyms||PSPH, Phosphoserine phosphatase Human, PSPase, PSP, EC 220.127.116.11, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, Phosphoserine phosphatase, L 3 phosphoserine phosphatase, O phosphoserine phosphohydrolase, Phosphoserine phosphatase deficiency, included,|
|Amino Acid Sequence||MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEE|
|Endotoxin Level||< 1.0 EU per 1 microgram of protein (determined by LAL method)|
|Molecular Weight||84 kDa (750 aa)|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 40mM Tris pH 8.0, 100mM NaCl, 4mM MgCl2, 2mM DTT, 40% glycerol|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_004568|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Moro-Furlani., AM., et al. (1980) Ann. Hum. Genet. 43, 323-333
Collet JF., et al. (1997) FEBS Lett. 408, 281-284
Kim HY, et al. (2002) J Biol Chem. 277, 46651-8.
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