PHPT1, 1-125aa, Human

Proteins, Recombinant
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Product Name PHPT1, 1-125aa, Human
Background PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms 14 kDa phosphohistidine phosphatase, bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10
Species Human
Molecular Weight 34.1 kDa (301aa) ) confirmed by MALDI-TOF
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_054891
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Srivastava S., et al. (2008) Proc Natl Acad Sci U S A. 105(38):14442-6.
van Vlierberghe P., et al. (2006) Leukemia. 20(7):1245-53.

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