Hi, have questions?
An ARP Customer Service Representative is happy to help!
|Product Name||Pin 1 (Peptidyl-prolyl cis/trans isomerase) Human, Recombinant|
|Background||Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.|
|Synonyms||PIN1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC 184.108.40.206, Rotamase Pin1, PPIase Pin1, DOD, UBL5, PIN1, PPIase, EC 220.127.116.11, Rotamase Pin1, PPIase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1|
|Amino Acid Sequence||MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE|
|Endotoxin Level||< 1.0 EU per 1 microgram of protein (determined by LAL method)|
|Molecular Weight||14 kDa (125 aa), confirmed by MALDI-TOF.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in Phosphate-Buffered Saline (pH 7.4)|
|Purity||95% by SDS-PAGE|
|NCBI Accession #||NP_006212|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Wulf GM., et al. (2002) J Biol. Chem. 277(50):47976-47979
Hamdane M., et al. (2002) J Mol Neurosci. 19(3); 275-287
Zheng H, et al. (2002) Nature. 419(6909) 853-857.
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.