POLR2J3, 1-115aa, Human

Proteins, Recombinant
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Product Name POLR2J3, 1-115aa, Human
Background DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms DNA-directed RNA polymerase II subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2
Species Human
Molecular Weight 19 kDa (171aa)
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M NaCl, 10% glycerol
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_001091084
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Palangat M., et al. (2004) J Mol Biol. 341:429-442
Uivari A., et al. (2006) Nat Struct Mol Biol. 13: 49-54.

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