| Product Name | PPIE (Cyclophilin E), 1-301aa, Human, Recombinant |
|---|---|
| Description | Cyclophilins possess the activity of peptidyl-prolyl cis-trans isomerase (PPIase) and are involved in cellular protein folding and protein interactions. PPIE contains two RNA binding domains at the N-terminal region and a PPIase domain at the C-terminal region. It was not known what cellular and physiological roles of PPIE. Recombinant human PPIE was expressed in E.coli and purified by conventional chromatography techniques. |
| Synonyms | CYP-33, Cyclophilin E, PPIase E, Rotamase E, peptidylprolyl isomerase E (cyclophilin E), PPIE, Peptidylprolyl isomerase E isoform 1 Cyclophilin 33, CYP33, EC 5.2.1.8 Peptidyl prolyl cis trans isomerase E, |
| Host | E.coli |
| Molecular Weight | 24.8 kDa (223 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATT KRVLYVGGLA EEVDDKVLHA AFIPFGDITD IQIPLDYETE KHRGFAFVEF ELAEDAAAAI DNMNESELFG RTIRVNLAKP MRIKEGSSRP VWSDDDWLKK FSGKTLEENK EEEGSEPPKA ETQEGEPIAK KARSNPQVYM DIKIGNKPAG RIQMLLRSDV VPMTAENFRC LCTHEKGFGF KGSSFHRIIP QFMCQGGDFT NHNGTGGKSI YGKKFDDENF ILKHTGPGLL SMANSGPNTN GSQFFLTCDK TDWLDGKHVV FGEVTEGLDV LRQIEAQGSK DGKPKQKVII ADCGEYV |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl pH8.0, 2mM EDTA |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Wang Y., et al. (2008) FEBS Lett. 582(5):835-9. Mi H., et al. (1996) FEBS Lett. 398:201-5. |
| Supplier | ARP |
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