PSMA3, 1-255aa, Human

Category: Proteins
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01-2011-3
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Product Name PSMA3, 1-255aa, Human
Description The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. Recombinant human PSMA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Proteasome (prosome, macropain) subunit, alpha type 3, HC8, MGC12306, MGC32631, PSC3
Host E.coli
Molecular Weight 44.1 kDa (392aa), confirmed by MALDI-TOF
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MSSIGTGYDL SASTFSPDGR VFQVEYAMKA VENSSTAIGI RCKDGVVFGV EKLVLSKLYE EGSNKRLFNV DRHVGMAVAG LLADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG YWGCAIGKAR QAAKTEIEKL QMKEMTCRDI VKEVAKIIYI VHDEVKDKAF ELELSWVGEL TNGRHEIVPK DIREEAEKYA KESLKEEDES DDDNM
Tag His-tag
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity > 95 % by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References Kristensen P., et al. (1995) Biochem Biophys Res Commun. 207(3):1059.
Wenzel T., et al. (1995) Nat Struct Biol. 2(3):199-204.
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