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|Product Name||PSMG4, 1-123aa, Human|
|Background||Proteasome assembly chaperone 4, also known as PSMG4, is a chaperone protein which promotes assembly of the 20S proteasome. It interacts with PSMG3, as well as directly alpha and beta subunits of the 20S proteasome but dissociates before the formation of half-proteasomes, probably upon recruitment of POMP. Recombinant human PSMG4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||Proteasome assembly chaperone 4, PAC4|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MEGLVVAAGG DVSLHNFSAR LWEQLVHFHV MRLTDSLFLW VGATPHLRNL AVAMCSRYDS IPVSTSLLGD TSDTTSTGLA QRLARKTNKQ VFVSYNLQNT DSNFALLVEN RIKEEMEAFP EKF|
|Molecular Weight||31.7kDa (274aa), confirmed by MALDI-TOF|
|Concentration||0.5mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl|
|Purity||> 95% by SDS-PAGE|
|NCBI Accession #||NP_001122063|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Le Tallec B., et al. (1998) Mol Cell. 17(4):660-74.
Ramos PC., et al. (1998) Cell. 20(4): 489-99.
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