RAMP3, 24-118aa, Human

Proteins, Recombinant
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Product Name RAMP3, 24-118aa, Human
Background RAMP3, also known as receptor activity modifying protein 3, is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). It is required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP3) protein, CRLR functions as an adrenomedullin receptor. Recombinant human RAMP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Receptor activity modifying protein 3, Receptor (calcitonin) activity modifying protein 3
Species Human
Molecular Weight 26.5 kDa (245aa) confirmed by MALDI-TOF
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_005847
Host E.coli
Tag His-tag
Storage Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.
Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Kuwasako K. et al. (2008) Biochem Biophys Res Commun. 377:109-113.
Roh J. et al. (2004) J Biol Chem. 279:7264-7274

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