RAP1B, 1-181aa, Human

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01-1858-1B
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Proteins, Recombinant
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Product Name RAP1B, 1-181aa, Human
Background RAP1B belongs to a superfamily of RAS-like small GTP-binding proteins involved in cell signaling. It possesses intrinsic GTPase activity and is ubiquitously expressed in mammalian tissues. The putative effector domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Recombinant human RAP1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Ras-related protein Rap-1b, DKFZp586H0723, K-REV, RAL1B
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINRKTP VPGKARKKSS C
Molecular Weight 26.1 kDa (237aa), confirmed by MALDI-TOF
Concentration 1.0 mg/ml (determined by BCA assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_056461
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Awasthi A., et al. (2010) J Exp Med. 207(9):1923-38.
Schoenwaelder SM., et al. (2010) J Biol Chem. 285(4):2886-96.

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