RBM17, 1-401aa, Human

Catalog
01-P2451
$0.00
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Proteins, Recombinant
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More Information
Product Name RBM17, 1-401aa, Human
Background RNA binding motif protein 17, also known as RBM17, is splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. This protein is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. This protein promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. Recombinant human RBM17 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms SPF45
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG GSSDDRQIVD
TPPHVAAGLK DPVPSGFSAG EVLIPLADEY DPMFPNDYEK VVKRQREERQ RQRELERQKE IEEREKRRKD RHEASGFARR PDPDSDEDED
YERERRKRSM GGAAIAPPTS LVEKDKELPR DFPYEEDSRP RSQSSKAAIP PPVYEEQDRP RSPTGPSNSF LANMGGTVAH KIMQKYGFRE
GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKDASKK SDSNPLTEIL KCPTKVVLLR NMVGAGEVDE DLEVETKEEC EKYGKVGKCV
IFEIPGAPDD EAVRIFLEFE RVESAIKAVV DLNGRYFGGR VVKACFYNLD KFRVLDLAEQ V
Molecular Weight 25.9kDa (240aa) confirmed by MALDI-TOF
(Molecular size on SDS-PAGE will appear higher)
Concentration 0.25 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,
20% glycerol, 1mM DTT
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_116294
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Lallena M.J., et al. (2002) Cell. 109:285-296
Carrascal M., et al. (2008) J. Proteome Res. 7:5167-5176

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