Recoverin, Human, Recombinant

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01-2230
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Proteins, Recombinant
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Product Name Recoverin, Human, Recombinant
Background Recoverin is a heterogeneously acylated calcium-binding and intracellular signal transduction protein in the photoreceptor cells of retina. Recoverin contains four EF-hands, of which two bind Ca. Ca-induced extrusion of the acyl group from a hydrophobic cleft in the protein drives the translocation of recoverin from solution to the disc membrane. Recently, recoverin is a detectable serologic protein that is expressed in patients with cancer-associated retinopathy, a paraneoplastic syndrome. Recombinant human recoverin was overexpressed in E. coli and purified by using conventional chromatography techniques
Synonyms RCVRN, RCV1, Recoverin, Cancer-associated retinopathy protein, Protein CAR, Recoverin, Cancer associated retinopathy protein, Cancer associated retinopathy protein antibody, CAR protein,
Species Human
Amino Acid Sequence MGNSKSGALS KEILEELQLN TKFSEEELCS WYQSFLKDCP TGRITQQQFQ SIYAKFFPDT DPKAYAQHVF RSFDSNLDGT LDFKEYVIAL HMTTAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVMAIF KMITPEDVKL LPDDENTPEK RAEKIWKYFG KNDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKMKNA
Molecular Weight 21.4 kDa (192 aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl pH7.5, 2mM EDTA, 1mMDTT
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_002894
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Sampath P., et al. (2004) Cancer Control. 11, 174-80. Erickson MA., et al. (1998) Proc. Natl. Acad. Sci. 95, 6474-9.

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