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|Product Name||RHOA, 1-190aa, Human|
|Background||RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila). Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||Ras homolog gene family, member A, ARH12, ARHA, RHO12, RHOH12|
|Amino Acid Sequence||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC|
|Molecular Weight||55.9 kDa(510aa), confirmed by MALDI-TOF|
|Concentration||1 mg/ml (determined by BCA assay)|
|Form||Liquid, in 20 mM Tris-HCl buffer (pH8.0) contain.ning 10% Glycerol|
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_001655|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Madaule P. et al. (1985) Cell. 41:31-40
Garrett M.D. et al. (1989) J. Biol. Chem. 264:10-13
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