RHOA, 1-190aa, Human

Catalog
01-1889-A
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Proteins, Recombinant
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Product Name RHOA, 1-190aa, Human
Background RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila). Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Ras homolog gene family, member A, ARH12, ARHA, RHO12, RHOH12
Species Human
Amino Acid Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC
Molecular Weight 55.9 kDa(510aa), confirmed by MALDI-TOF
Concentration 1 mg/ml (determined by BCA assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH8.0) contain.ning 10% Glycerol
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_001655
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Madaule P. et al. (1985) Cell. 41:31-40
Garrett M.D. et al. (1989) J. Biol. Chem. 264:10-13

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