| Product Name | RND3, 1-241aa, Human |
|---|---|
| Description | Rho-related GTP-binding protein RhoE, also known as RND3, is a small GTPase of the Ras superfamily that regulate the organization of the actin cytoskeleton in response to extracellular growth factors. RND3 activity leads to a decrease in stress fibers and increased cell migration, at least in part through regulation of the Rho-dependent kinase ROCK1. Also, RND3 has been implicated in inhibition of DNA-damage induced apoptosis and cell cycle arrest. Recombinant human RND3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | ARHE, memB, Rho8, RhoE, Rho-related GTP-binding protein RhoE |
| Host | E.coli |
| Molecular Weight | 38.9 kDa (372aa) confirmed by MALDI-TOF (Real molecular weight on SDS-PAGE will be shift up) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MKERRASQKL SSKSIMDPNQ NVKCKIVVVG DSQCGKTALL HVFAKDCFPE NYVPTVFENY TASFEIDTQR IELSLWDTSG SPYYDNVRPL SYPDSDAVLI CFDISRPETL DSVLKKWKGE IQEFCPNTKM LLVGCKSDLR TDVSTLVELS NHRQTPVSYD QGANMAKQIG AATYIECSAL QSENSVRDIF HVATLACVNK TNKNVKRNKS QRATKRISHM PSRPELSAVA TDLRKDKAKS C |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.2M Nacl, 1mM DTT, 10% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Riento K., et al. (2005) Mol Cell Biol. 23:4219-29. Boswell S A., et al. (2007) J Biol Chem. 282:4850-8 |
| Supplier | ARP |
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