SDC1, 18-254aa, Human

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01-P1467
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Proteins, Recombinant
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Product Name SDC1, 18-254aa, Human
Background Syndecan-1 precursor, also known as SDC1, is a transmembrane heparin sulphate proteoglycan which is made up of one core protein and five glycosaminoglycan. SDC1 is expected to play a role in cell adhesion. It is expressed on the surface of pre-B cells and plasma cells but is absent from mature B cells. It is a selective marker for B cell lymphoblastic leukemia and lymphoplasmocytoid leukemia. Recombinant human SDC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Syndecan-1 precursor, CD138, SDC, SYND1, syndecan
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSHMQPALP QIVATNLPPE DQDGSGDDSD NFSGSGAGAL QDITLSQQTP STWKDTQLLT AIPTSPEPTG LEATAASTST LPAGEGPKEG EAVVLPEVEP GLTAREQEAT PRPRETTQLP TTHQASTTTA TTAQEPATSH PHRDMQPGHH ETSTPAGPSQ ADLHTPHTED GGPSATERAA EDGASSQLPA AEGSGEQDFT FETSGENTAV VAVEPDRRNQ SPVDQGATGA SQGLLDRKEV LG
Molecular Weight 35.4kDa (313aa), confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_002988
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References O’Connell FP., et al. (2004) Am J Clin Pathol. 121(2):254-63-21531.
Rawstron AC., et al. (2006) Curr Protoc Cytom. 6: Unit6.23.

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