SEPT2, 1-361aa, Human

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01-1764-2
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Proteins, Recombinant
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Product Name SEPT2, 1-361aa, Human
Background Septin2 (SEPT2) is a GTPase that is required for cytokinesis and associated with exocytosis. This protein can hetero-oligomerize with Septin6, 7 and also plays a role in the organization of new growth in organisms. It is associated with a Tau-based paired helical filament core and may contribute to the formation of neurofibrillary tangle as integral constituents of paired helical filaments. Recombinant human Septin2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Septin-2, hNedd5, KIAA0158, DIFF6, Pnutl3, SEPTIN2
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MSKQQPTQFI NPETPGYVGF ANLPNQVHRK SVKKGFEFTL MVVGESGLGK STLINSLFLT DLYPERVISG AAEKIERTVQ IEASTVEIEE RGVKLRLTVV DTPGYGDAIN CRDCFKTIIS YIDEQFERYL HDESGLNRRH IIDNRVHCCF YFISPFGHGL KPLDVAFMKA IHNKVNIVPV IAKADTLTLK ERERLKKRIL DEIEEHNIKI YHLPDAESDE DEDFKEQTRL LKASIPFSVV GSNQLIEAKG KKVRGRLYPW GVVEVENPEH NDFLKLRTML ITHMQDLQEV TQDLHYENFR SERLKRGGRK VENEDMNKDQ ILLEKEAELR RMQEMIARMQ AQMQMQMQGG DGDGGALGHH V
Molecular Weight 30.7kDa (269aa), confirmed by MALDI-TOF
Concentration 0.5mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 100mM NaCl, 0.1mM PMSF
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # AAH14455
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Low C, et al.. (2006) J Biol Chem. 281(41):30697-706.
Kremer BE, et al. (2007) Cell. 130(5):837-50.

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