SNAP-25 (Synaptosomal-associated protein 25kDa), C.elegans Recombinant

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01-2215-2
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Proteins, Recombinant
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Product Name SNAP-25 (Synaptosomal-associated protein 25kDa), C.elegans Recombinant
Background SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release . The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane. SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.
Synonyms SNAP25, SNAP-25, Synaptosomal-associated protein 25kDa, FLJ23079, RIC-4, RIC4, SEC9, SNAP 25, bA416N4.2, dJ1068F16.2, SUP, resistance to inhibitors of cholinesterase 4 homolog, super protein, synaptosomal-associated 25 kDa protein, synaptosomal-associated protein 25, RIC 4
Species Human
Amino Acid Sequence MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERC EGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQP RITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK
Molecular Weight 13.3 kDa (127 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
Concentration 1 mg/ml (determined by BCA assay)
Form Liquid, in 20 mM Tris-HCl buffer (pH 7.5) containing 0.1 M NaCl
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_003072.2
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Saifee O., et al. (1998) Mol.Biol.Cell 9(6);1235-52.
Yang Y., et al. (2000) . 275(38);29482-7.

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