Synaptobrevin 1, 1-91aa, Human, Recombinant

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01-2303
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Proteins, Recombinant
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Product Name Synaptobrevin 1, 1-91aa, Human, Recombinant
Background Synaptobrevin 1(Vehicle-associated membrane, VAMP1) is one of the key proteins in the SNARE complex which is involved in regulated exocytosis. Synaptobrevin1 binds to t-SNAREs, syntaxin (STX) and SNAP25, after the fusion of synaptic vesicles to plasma membrane. Recombinant human Synaptobrevin1, fused to His- tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms VAMP1, SYB1, Synaptobrevin 1, VAMP-1, Synaptobrevin-1, Vesicle-associated membrane protein 1, Vesicle-associated membrane protein 1 isoform 1 DKFZp686H12131, SYB 1, Synaptobrevin1, VAMP 1, Vesicle associated membrane protein 1, Vesicle associated membrane protein 1 synaptobrevin 1.
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MSAPAQPPAE GTEGTAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIIRV NVDKVLERDQ KLSELDDRAD ALQAGASQFE SSAAKLKRKY W
Molecular Weight 18 kDa (158 aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 50 mM HEPES (pH7.5) 150mM NaCl, 1mM DTT, 10%glycerol.
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_055046
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Nystuen AM., et al. (2007) Neurogenetics. 8:1-10.
Pennuto M., et al. (2003) Mol Biol Cell. 14:4909-19.

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